Facilities

Directed by Mohamed Al-Shabrawey, MD, Ph.D., the Cell and Molecular Biology Module provides training and access to Cytation5 Cell Imaging Multimode Reader, 颗粒- metrix ZetaView quat - nta纳米颗粒跟踪-视频显微镜和SeahorseXFe96分析仪高质量的活细胞分析.

To learn more and book equipment usage, click here.

ERI成立了Virginia and Clarence Clohset儿童视网膜研究实验室(PRRL) in 2011, 哪个实验室被认为是国内唯一一个专门从事儿童视网膜研究的实验室. The PRRL is directed by Dr. Kenneth Mitton. ERI clinical faculty Dr. Kimberly Drenser and Dr. Antonio Capone Jr. are also associated with the laboratory. PRRL在密歇根州的prrrf和卡尔斯基金会的财政支持下运作.

$480,000 in funding to construct the laboratory space was donated by the Pediatric Retinal Research Foundation, 它正在努力消除因早产和视网膜疾病导致的儿童失明和视力低下. 该基金是通过两个捐赠家庭- - Clohsets和Bergquist家庭- -以及一些个人捐助者的支持作出捐助的. Additional funding from the PRRF, the NEI/NIH, 卡尔斯基金会为ERI研究实验室的几个研究项目提供了设备和试剂. Furthermore, Dr. Drenser (Associated Retinal Consultants) and Dr. Mitton合作并发表了关于Norrin及其在视网膜血管发育中的作用的研究，使用动物模型和来自人类器官捐赠视网膜的血管细胞. 患有诺里病的人类患者编码诺里蛋白的基因发生了变化，这导致婴儿视网膜缺乏血管, causing blindness from an early age. Norrin是一种特殊的生长因子，与视网膜血管细胞上的受体蛋白结合. 其他患有不同遗传病的患者称为FEVR(家族性渗出性玻璃体视网膜病变)，他们的基因编码一种或多种蛋白质，形成Norrin受体复合物. 这些基因分别被命名为FZD4 (frizzled4)、TSPAN12 (tfour - spannin -12)和LRP5.

Norrie Disease, FEVR, Persistent Fetal Vascular Syndrome, 和视网膜裂是影响幼儿和成人的相对罕见的致盲疾病. 视网膜裂患者的视网膜非常脆弱，由于RS基因的变化，会出现撕裂和分层, 它编码一种叫做rs蛋白的胶状蛋白质，这种蛋白质通常将视网膜层连接在一起. In the United States rare diseases that affect less than 200,000人被称为“孤儿”疾病，罕见疾病往往得不到医学研究的关注.

Fortunately, 密歇根的视网膜副顾问是世界知名的罕见疾病专家医生，儿童视网膜研究基金会已经将这些孤儿疾病作为他们研究支持的目标. By partnering with Oakland University, 基础科学研究有助于了解这些疾病并开发治疗方法.

PRRL以多种方式支持ERI在365英国上市官网的转化医学研究任务.

• Live retinal imaging and ERG testing for mouse and rat eye research models. This includes digital imaging of the retina and fluorescein angiography, which are methods used on human eye patients too. The PRRL also has Optical Coherence Tomography imaging, a way to see the layers of the living retina, painlessly, in a living eye.
• Cell culture, 用于从捐赠给医学研究的人类视网膜中提取的初级视网膜血管细胞. These cells enable the direct study of the effects of growth factors, drugs, 高葡萄糖(糖尿病)对形成人眼重要血液视网膜屏障的细胞造成影响.
• Next Generation Sequencing (NGS), DNA sequencing to test genes for changes that may cause Norrie Disease, FVER. 2019年夏天，PRRL/ERI启动了365英国上市官网第一个正在进行的DNA测序研究服务, which focuses on orphan pediatric retinal diseases. Dr. Mitton, Dr. Drenser, 技术专家温迪·戴利(Wendy Dailey)使用Illumina公司开发了一种定制的靶向DNA测序测试面板, USA) technology. Dr. Mitton and Wendy Dailey, working in the PRRL, 开发了一个8基因测试面板，以正确检测致病变化所需的准确性对基因进行排序. Aware of the fact that US health insurance does not cover such testing, 该团队希望将基于测序的分析成本从一两个基因的几千美元降低到八个基因的250美元左右. 截至2023年1月，已经对80多名出血热患者进行了检测，并在10个家庭中确定了这种疾病的分子原因. PRRL还为365英国上市官网理科和医科学生提供了应用人类遗传学教育的机会.

The Ocular Structure and Imaging (OSI) facility is supervised by Andrew Goldberg, Ph.D., and is staffed by a full-time Ph.D.-level digital imaging associate supported by the university. 它提供仪器和专业知识，以支持电子和光学显微镜研究.

Directed by Amany Tawfik, M.D., M.Sc., 该模块允许通过荧光素血管造影和光学相干断层扫描(OCT)研究视网膜结构和血管的变化。. 它还允许使用激光源诱导实验性脉络膜新生血管(凤凰科技集团).

Retinal Function Assessment Module, directed by Dao Qi Zhang, Ph.D., contains:

1. Celeris电生理系统:它提供全视野暗适应和光适应视网膜电图(ERG)测试, flash visual evoked potential (VEP) testing, simultaneous ERG and VEP protocol provided, both eyes can be tested individually and automatically, 还有一种专门设计的麻醉机可以安全地给老鼠注射异氟醚.
2. 脑力学验光- at系统:为小实验动物(大鼠、小鼠)提供实时的光动力学反应行为测试, 空间频率和对比灵敏度测试以及手动和自动试验控制和跟踪评估.
3.  NeurOptics A-2000小动物瞳孔测量系统:提供小实验动物(大鼠和小鼠)的实时瞳孔评估, the binocular dual-camera system measures both eyes at once, four ultra-bright color light stimuli (white, green, blue, and red), and light intensities defined in lux or pure radiometric units (W/m2), stimulates direct, consensual, or both eyes simultaneously and automatic tracking and pupil detection.